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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Tumor protein p53 |
| Gene Symbol: | TP53 |
| Synonyms: | P53; BCC7; LFS1; BMFS5; TRP53 |
| Gene ID: | 7157 |
| UniProt ID: | P04637 |
| Immunogen: | A synthesized peptide derived from human p53 |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene TP53 encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Images
 | Western blotting analysis using anti-tumor protein p53 antibody (Cat#71136). Total cell lysates (30 μg) from HAP-1 cells were loaded and separated by SDS-PAGE. The blot was incubated with anti-tumor protein p53 antibody (Cat#71136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-tumor protein p53 antibody (Cat#71136). Total cell lysates (30 μg) from HAP-1 cells were loaded and separated by SDS-PAGE. The blot was incubated with anti-tumor protein p53 antibody (Cat#71136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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