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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | tripartite motif containing 7 |
| Gene Symbol: | TRIM7 |
| Synonyms: | GNIP; RNF90 |
| Gene ID: | 81786 |
| UniProt ID: | Q9C029 |
| Clone ID: | 25GB690 |
| Immunogen: | Recombinant protein of human TRIM7 |
| Dilution: | WB 1:400-1:2,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1, a B-box type 2, and a coiled-coil region. The protein localizes to both the nucleus and the cytoplasm, and may represent a participant in the initiation of glycogen synthesis. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-TRIM7 antibody (Cat#71166). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM7 antibody (Cat#71166, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-TRIM7 antibody (Cat#71166). TRIM7 expression in wild type (WT) and TRIM7 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TRIM7 antibody (Cat#71166, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TRIM7 expression in HeLa cells using anti-TRIM7 antibody (Cat#71166, 1:2,000). Green, isotype control; red, TRIM7. |
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