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Cytokeratin 17 (KRT17) Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | keratin 17 |
| Gene Symbol: | KRT17 |
| Synonyms: | PC; K17; PC2; 39.1; CK-17; PCHC1; Cytokeratin 17 (KRT17) |
| Gene ID: | 3872 |
| UniProt ID: | Q04695 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 133-432 of human Cytokeratin 17 (KRT17) (NP_000413.1). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.59 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-KRT17 antibody |
Background
This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex.
Images
 | Western blot analysis of various lysates using Cytokeratin 17 (KRT17) Rabbit pAb (A0123) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from Mouse stomach, using Cytokeratin 17 (KRT17) Rabbit pAb (A0123) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Confocal immunofluorescence analysis of HeLa cells using Cytokeratin 17 (KRT17) Rabbit pAb (A0123) at dilution of 1:200. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using Cytokeratin 17 (KRT17) Rabbit pAb (A0123) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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