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Cytokeratin 7 (KRT7) Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | keratin 7 |
| Gene Symbol: | KRT7 |
| Synonyms: | K7; CK7; SCL; K2C7; Cytokeratin 7 (KRT7) |
| Gene ID: | 3855 |
| UniProt ID: | P08729 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human Cytokeratin 7 (KRT7) (NP_005547.3). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.12 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-KRT7 antibody |
Background
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
Images
 | Western blot analysis of lysates from HepG2 cells, using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Western blot analysis of lysates from Rat lung, using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of human lung cancer using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse bronchus using Cytokeratin 7 (KRT7) Rabbit pAb (A12004) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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