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Cytokeratin 9 (KRT9) Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | keratin 9 |
| Gene Symbol: | KRT9 |
| Synonyms: | K9; CK-9; EPPK; Cytokeratin 9 (KRT9) |
| Gene ID: | 3857 |
| UniProt ID: | P35527 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-450 of human Cytokeratin 9 (KRT9) (KRT9) (NP_000217.2). |
| Dilution: | WB 1:1000-1:5000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.42 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-KRT9 antibody |
Background
This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma.
Images
 | Western blot analysis of lysates from Mouse brain, using Cytokeratin 9 (KRT9) Rabbit pAb (A10119) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of paraffin-embedded human skin using Cytokeratin 9 (KRT9) Rabbit pAb (A10119) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
 | Immunofluorescence analysis of paraffin-embedded mouse skin using Cytokeratin 9 (KRT9) Rabbit pAb (A10119) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
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