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| Reactivity: | Human, Mouse |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | LIM domain and actin binding 1 |
| Gene Symbol: | LIMA1 |
| Synonyms: | EPLIN; LDLCQ8; SREBP3; LIMA1 |
| Gene ID: | 51474 |
| UniProt ID: | Q9UHB6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 560-759 of human LIMA1 (NP_057441.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.30 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-LIMA1 antibody |
Background
This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated.
Images
 | Western blot analysis of lysates from A-549 cells, using LIMA1 Rabbit pAb (A11682) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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