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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | lysozyme |
| Gene Symbol: | LYZ |
| Synonyms: | LZM; LYZF1; Lysozyme (LYZ) |
| Gene ID: | 4069 |
| UniProt ID: | P61626 |
| Clone ID: | 2Y5E4 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Lysozyme (LYZ) (NP_000230.1). |
| Dilution: | WB 1:30000-1:120000; IHC 1:1000-1:10000; IF/IC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.23 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-LYZ antibody |
Background
This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk,and is also present in spleen,lung,kidney,white blood cells,plasma,saliva,and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.
Images
 | Western blot analysis of various lysates using Lysozyme (LYZ) Rabbit mAb (A10972)at 1:60000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): HeLa Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Lysozyme (LYZ) Rabbit mAb (A10972) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Lysozyme (LYZ) Rabbit mAb (A10972) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Lysozyme (LYZ) Rabbit mAb (A10972) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Lysozyme (LYZ) Rabbit mAb (A10972) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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