Lentivirus Transduction Enhancer I (1 ml)

Lentivirus Transduction Enhancer I is a novel chemical reagent that increases lentiviral transduction efficiency into in vitro systems. Normally, incubation of lentivirus particles in cells in the presence ofpolybrene is generally efficient in transducing many cell types. However, lentiviruses are typically attenuated by cellular antiviral defense mechanism, which limits their transducing efficiency. Lentivirus Transduction Enhancer I suppresses the cellular anti-viral state by mimicking the activity of viral virulence gene products, and thus significantly increases lentivirus transducing efficiency by as high as10-fold.

Lentivirus Transduction Enhancer I is most effective when added to cell culture media at the time of transduction. Recommended working concentration ranges from 1:100 to 1:50. However, the working concentration is highly cell line-dependent. Lower or higher dilution ratio may be required to optimize the effect.