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| Key Features: | • Complete bisulfite conversion of DNA in less than 2 hours. • Desulphonation and recovery of bisulfite-treated DNA with a spin column. • Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc. |
| Description: | LiDirectTM DNA Methylation Kit integrates DNA denaturation and bisulfite conversion processes into one-step. Also, the kit has been streamlined for high yield recovery of DNA following DNA bisulfite conversion. The DNA Methylation Kit provides innovative in-column desulphonation technology that eliminates cumbersome DNA precipitation steps while providing researchers consistent results every time. The kit is optimized to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc. |
| Applications: | High-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. |
| Technical Specifications: | • Conversion: >99% • Elution Volume: ≥20 μl • Input: 500 pg – 2 μg of DNA • Process Time: 2 hours • Recovery: >75% |
| Sample Source: | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Component: | 1. CT Conversion Reagent: 5 Tubes 2. Dilution Buffer: 1.5 ml 3. Dissolving Buffer: 500 µl 4. Binding Buffer: 30 mL 5. Wash Buffer: 6 ml 6. Desulphonation Buffer: 10 ml 7. Elution Buffer: 1.2 ml 8. Mini Column: 50 9. 2 ml Collection Tube: 50 |
| Storage: | Room Temperature |
| Documents: | Manual |
