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| Key Features: | • Excellent silencing at low concentrations with only 1.0 nM siRNA/miRNA mimics • Minimizing non-specific and off target effects in the cell • One tube reaction • Best for broad mammalian cells • Very low cytotoxicity |
| Description: | LiFectRNA™ Transfection Reagent is a novel biodegradable non-liposomal siRNA/miRNA & DNA delivery tool. With our proprietary pH Dependent Conformational Change (PDCC) technology, the biodegradable transfection reagent was formulated by addition of pre-screened hydrophobic groups to its side chain, making LiFectRNA™ Reagent a versatile and most powerful gene delivery tool in the market. LiFectRNA™ Reagent have been validated to effectively and reproducibly transfect single siRNA/miRNA or co-transfect DNA/siRNA or DNA/miRNA to variety of mammalian cells. |
| Applications: | 1. Single siRNA transfection or DNA/siRNA co-transfection to variety of mammalian cells.. 2. Single miRNA, miRNA mimics or inhibitors transfection or DNA/miRNA or DNA/miRNA mimics co-transfection to variety of mammalian cells.. |
| Component: | 1. LiFectRNA™ Reagent, 1.0 mL, sufficient for ~2,000 reactions based on transfecting 5.0 pmol siRNA or miRNA mimics in 24-well plate. 2. LiFectRNA™ Transfection Buffer (5×), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution. |
| Storage: | Store at 4 °C for LiFectRNA™ Reagent and RT for LiFectRNA™ Transfection Buffer (5×). If stored properly, the product is stable for 12 months or longer. |
| Documents: | Manual |
Case Study
Excellent silencing of endogenously expressed KIF11 (also known as Eg5) in HEK293 cells with 0.5 µl of LiFectRNA™ reagent and 5.0 pmol Eg5 siRNA per well of 24-well plate. KIF11 (also known as Eg5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LiFectRNA™ reagent effectively delivers Eg5 siRNA (final 10 nM, right panel) to HEK293 cells at only 0.5 µl per well of 24-well plate vs. negative control (final 10 nM. left panel). Compared with negative control (left panel), phenotype of "rounded-up" 293 cells were visualized 24 hours post transfection (right panel) with a Nikon microscope.
LiFectRNA™ Transfection Reagent knocked down endogenous lamin A/C gene expression in Hela cells. A siRNA targeting lamin A/C gene (right panel) and a sham siRNA (left panel) were introduced into Hela cells (final 1.0 nM) by LiFectRNA™ Transfection Reagent. Lamin A/C gene silencing was monitored 24h post transfection by immunofluorescence. Lamin A/C was probed with a mouse monoclonal lamin A/C specific antibody followed by addition of FITC-conjugated anti-mouse antibodies. Quantitative analysis showed that lamin siRNA at 1.0 nM delivered by LiFectRNA™ Transfection Reagent knocked down 94% endogenously expressed lamin A/C in Hela cells.
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