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| Key Features: |
• Rapid: Purification of plasmid DNA in less than 30 minutes. • Safe: No Phenol/chloroform extractions. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: |
LiPureTM Magbead Plasmid Mini Kit is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-6 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with magnetic beads technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing. |
| Technical Specifications: |
• Applications: Plasimd DNA isolation from 1-6 ml bacterial cultures • Sample Source: LB culture • Sample Input: 1-6 ml high-copy plasimd, 10 ml low-copy plasimd • Elution Volume: 30-100 μl • Technology: Magnetic Bead |
| Component: |
1. Buffer P1: 60 ml 2. Buffer P2: 60 ml 3. Buffer N3: 80 ml 4. Buffer PW : 2x25 ml 5. Buffer EB: 25 ml 6. RNase A (10 mg/ml): 600 µl 7. MagBinding Beads: 10 ml |
| Storage: |
Buffer at 15-30°C; RNase A at -20°C; MagBinding Beads at 2-8°C |
| Documents: |
Manual |
Case Study
Table 1. pUC18 plasmid DNA yield and quality using LiPureTM Magbead Plasmid Mini Kit
