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| Key Features: | • Higher sensitivity: 100 times more sensitive than NanoDrop. • Broad dynamic range: over four orders of magnitude. • More accuracy: unaffected by the presence of proteins, free nucleotides. • Easy to use: mix-and-read format. • Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit®. |
| Description: | LiQuant™ dsDNA BR Assay Kit provides a simple, sensitive, and accurate quantitation for dsDNA.The kit contains concentrated assay reagent, dilution buffer, and pre-diluted dsDNA standards. The assay kit is highly selective for dsDNA, and highly reliable in detecting dsDNA ranging from 2 to 1000 ng, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The assay is performed at room temperature. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 50 µl is acceptable), and read the fluorescenceusing fluorescence plate reader or Fluorometer such as Qubit® or Quantus™ Fluorometer. The kit is well tolerated to common contaminants such as proteins, salts, solvents and detergents. |
| Specifications: | 1. Sample Type: dsDNA 2. Platform: Fluorescence plate reader or fluorometer 3. Assay: dsDNA Quantitation, broad range 4. Linear Range: 2-1000 ng dsDNA |
| Applications: | dsDNA quantitation of genomic and miniprep DNA samples |
| Component: | 1. LiQuant™ dsDNA Reagent : 1 ml 2. LiQuant™ dsDNA Buffer: 200 ml 3. dsDNA Standard #1: 1 ml 4. dsDNA Standard #2: 1 ml |
| Storage: | At 2-8°C and protect from light |
| Download: | Manual |
Left: Quantitation of dsDNA with LiQuant™ dsDNA Assay Kit using fluorescence plate reader. Right: Quantitation of dsDNA with LiQuant™ dsDNA Assay Kit using Qubit® Fluorometer.