LiScript™ First-Strand cDNA Synthesis Kit (+gDNA wiper) (100 rxn)

• Rapid genomic DNA deletion: With the gDNA Eraser, it takes only 2 minutes to remove genomic DNA.

• Rapid reverse transcription: It takes only 15 minutes to obtain the first strand of cDNA.

• High sensitivity: pg of total RNA or mRNA can be used as template.

• High efficiency of reverser transcription efficiency: the novel mutation site enhancers the activity of the enzyme, to increasethe yield of cDNA.

This product is a kit for reverse transcription after removal of genomic DNA. The kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Eraser, the sample processed by gDNA Eraser can be directly used for reverse transcription reaction to synthesize cDNA.

LiScript™ First-Strand cDNA Synthesis Kit (+gDNA wiper) contains a novel high-performance reverse transcriptase LiScript™. The novel mutation site greatly enhances the transcriptional activity of the enzyme. The efficiency and yield of cDNA first-strand synthesis are higher, and the first strand of cDNA can be synthesized using pg total RNA or mRNA. If the cDNA is used for downstream qPCR, the reverse transcription reaction can be completed at 42°C for 15 minutes. This kit is suitable for the synthesis of first-strand cDNA and subsequent RT-PCR, RT-qPCR, and construction of full-length cDNA libraries.

• PCR

• qPCR

• PCR Cloning

1. gDNA Eraser: 50 μl
2. 10× gDNA Erase Buffer: 120 μl
3. LiScript, 200 U/μl: 100 μl
4. 5× ScriptRT Buffer: 500 μl
5. Primer Mix: 120 μl
6. RNase-Free Water: 2×1 ml