Key Feature: | • 5 minutes cDNA synthesis • cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates. • Passive reference dyes are provided for different qPCR instruments. |
Description: | LiScript™ One Step Probe RT-qPCR Kit combines the first-strand cDNA synthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. LiScript™ Probe One Step RT-qPCR Kit contains all the necessary reagents for cDNA synthesis and qPCR except probe, total RNA/mRNA template and gene specific primers. |
Application: | • Multiple copy and low copy gene detection • GC-rich or complex secondary structure RNA template • Viral RNA and trace RNA detection |
Component: | 1. RNase free ddH2O: 1.25 ml×2 2. 2× One Step Probe Mix: 1.25 ml×2 3. One Step Probe Enzyme Mix: 250 μl 4. 50× ROX Reference Dye 1: 100 μl 5. 50× ROX Reference Dye 2: 100 μl |
Storage: | At -20°C |
Download: | Manual |