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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | methylcrotonyl-CoA carboxylase subunit 1 |
| Gene Symbol: | MCCC1 |
| Synonyms: | MCCA; MCC-B; MCCCalpha; MCCC1 |
| Gene ID: | 56922 |
| UniProt ID: | Q96RQ3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 640-725 of human MCCC1 (NP_064551.3). |
| Dilution: | WB 1:1000-1:5000; IHC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 2.1 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MCCC1 antibody |
Background
This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.
Images
 | Western blot analysis of lysates from T-47D cells, using MCCC1 Rabbit pAb (A10020) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of various lysates, using MCCC1 Rabbit pAb (A10020) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MCCC1 Rabbit pAb (A10020) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat liver using MCCC1 Rabbit pAb (A10020) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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