GNT-V/MGAT5 Rabbit pAb (20 μl)

GNT-V/MGAT5 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A10567
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse    
Applications: WB, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase
Gene Symbol: MGAT5
Synonyms: GNT-V; GNT-VA; MGAT5A; glcNAc-T V; GNT-V/MGAT5
Gene ID: 4249
UniProt ID: Q09328
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 627-741 of human GNT-V/MGAT5 (NP_002401.1).
Dilution: WB 1:100-1:500
Purification Method: Affinity purification
Concentration: 0.22 mg/ml
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-MGAT5 antibody




Background

The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies.




Images

MGAT5 antibodyWestern blot analysis of various lysates using GNT-V/MGAT5 Rabbit pAb (A10567) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of various lysates using GNT-V/MGAT5 Rabbit pAb (A10567) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.



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