Reactivity: | Human, Mouse |
Applications: | WB, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase |
Gene Symbol: | MGAT5 |
Synonyms: | GNT-V; GNT-VA; MGAT5A; glcNAc-T V; GNT-V/MGAT5 |
Gene ID: | 4249 |
UniProt ID: | Q09328 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 627-741 of human GNT-V/MGAT5 (NP_002401.1). |
Dilution: | WB 1:100-1:500 |
Purification Method: | Affinity purification |
Concentration: | 0.22 mg/ml |
Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-MGAT5 antibody |
Background
The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies.
Images
![]() | Western blot analysis of various lysates using GNT-V/MGAT5 Rabbit pAb (A10567) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
![]() | Western blot analysis of various lysates using GNT-V/MGAT5 Rabbit pAb (A10567) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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