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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | MIS12 kinetochore complex component |
| Gene Symbol: | MIS12 |
| Synonyms: | MTW1; hMis12; KNTC2AP; 2510025F08Rik; MIS12 |
| Gene ID: | 79003 |
| UniProt ID: | Q9H081 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human MIS12 (NP_076944.1). |
| Dilution: | WB 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.64 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-MIS12 antibody |
Background
Involved in attachment of mitotic spindle microtubules to kinetochore and kinetochore assembly. Located in kinetochore and nucleus. Part of MIS12/MIND type complex.
Images
 | Western blot analysis of various lysates using MIS12 Rabbit pAb (A15508) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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