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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | mitochondrial ribosomal protein L28 |
| Gene Symbol: | MRPL28 |
| Synonyms: | p15; MAAT1; MRPL28 |
| Gene ID: | 10573 |
| UniProt ID: | Q13084 |
| Clone ID: | 3B9F2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MRPL28 (NP_006419.2). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.8 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MRPL28 antibody |
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.
Images
 | Western blot analysis of various lysates using MRPL28 Rabbit mAb (A0452) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates using MRPL28 Rabbit mAb (A0452) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg MRPL28 antibody (A0452). Western blot was performed from the immunoprecipitate using MRPL28 antibody (A0452) at a dilution of 1:1000. |
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