| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 28S ribosomal protein S31, mitochondrial |
Synonyms: | Imogen 38, IMOGN38, MRP S31, S31mt |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MRPS31 |
Uniprot ID: | Q61733 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 44 kDa / 37 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 3000-1: 4000 | liver |
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.
Images
| Western blot analysis of MRPS31 (GB115006) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |