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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | mechanistic target of rapamycin kinase |
| Gene Symbol: | MTOR |
| Synonyms: | SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR |
| Gene ID: | 2475 |
| UniProt ID: | P42345 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 2400-2500 of human mTOR (NP_004949.1). |
| Dilution: | WB 1:100-1:500; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.86 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-MTOR antibody |
Background
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.
Images
 | Western blot analysis of various lysates using mTOR Rabbit pAb (A11355) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb (A11355) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using mTOR Rabbit pAb (A11355) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug mTOR Rabbit pAb (A11355 1:50). Western blot was performed from the immunoprecipitate using mTOR Rabbit pAb (A2445) at a dilition of 1:500. |
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