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| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | myogenic differentiation 1 |
| Gene Symbol: | MYOD1 |
| Synonyms: | PUM; MYF3; MYOD; CMYP17; bHLHc1; MYODRIF; MyoD1 |
| Gene ID: | 4654 |
| UniProt ID: | P15172 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 226-300 of human MyoD1 (NP_002469.2). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.23 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MYOD1 antibody |
Background
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Images
 | Western blot analysis of lysates from wild type (WT) and 293T cells transfected with MyoD1 using MyoD1 Rabbit pAb (A0671) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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