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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | neutrophil cytosolic factor 2 |
| Gene Symbol: | NCF2 |
| Synonyms: | NCF-2; NOXA2; P67PHOX; P67-PHOX; NOXA2/p67phox |
| Gene ID: | 4688 |
| UniProt ID: | P19878 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NOXA2/p67phox (NP_000424.2). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:100-1:400 |
| Purification Method: | Affinity purification |
| Concentration: | 1.61 mg/ml |
| Buffer: | PBS with 0.09% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-NCF2 antibody |
Background
This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.
Images
 | Western blot analysis of various lysates using NOXA2/p67phox Rabbit pAb (A1178) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of Human spleen tissue using NOXA2/p67phox Rabbit pAb (A1178) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. |
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