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| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | NCK adaptor protein 1 |
| Gene Symbol: | NCK1 |
| Synonyms: | NCK; nck-1; NCKalpha; NCK1 |
| Gene ID: | 4690 |
| UniProt ID: | P16333 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 58-377 of human NCK1 (NP_006144.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.69 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NCK1 antibody |
Background
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Images
 | Western blot analysis of lysates from mouse brain, using NCK1 Rabbit pAb (A0999) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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