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| Reactivity: | Human |
| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | nuclear factor kappa B subunit 1 |
| Gene Symbol: | NFKB1 |
| Synonyms: | KBF1; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1; NF-kappabeta; NFKB1 |
| Gene ID: | 4790 |
| UniProt ID: | P19838 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of NFKB1 (NP_001158884.1). |
| Dilution: | WB 1:100-1:500; IHC 1:50-1:200; IF/IC 1:20-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 0.87 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NFKB1 antibody |
Background
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
Images
 | Western blot analysis of lysates from Raji cells, using NFKB1 Rabbit pAb (A11160) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil using NFKB1 Rabbit pAb (A11160) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of MCF7 cells using NFKB1 Rabbit pAb (A11160).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. |
 | Immunoprecipitation analysis of 150 μg extracts of MCF-7 cells using 3 μg NFKB1 antibody (A11160). Western blot was performed from the immunoprecipitate using NFKB1 antibody (A11160) at a dilution of 1:500. |
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