OXCT2 Rabbit pAb (20 μl)

OXCT2 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A14920
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IF/IC, ELISA    
Host Species:   Rabbit    
Clonality:   Polyclonal antibody    
Gene Name:   3-oxoacid CoA-transferase 2
Gene Symbol:   OXCT2
Synonyms:   SCOTT; FKSG25; OXCT2
Gene ID:   64064
UniProt ID:   Q9BYC2
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 430-510 of human OXCT2 (NP_071403.1).
Dilution:   WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   3.8 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-OXCT2 antibody




Background

The protein encoded by this gene catalyzes the transfer of a CoA group from succinate to acetoacetate and is an important enzyme in ketone body catabolism. The encoded protein localizes to the mitochondrion. This gene is intronless, and a pseudogene of this gene is located elsewhere on chromosome 1.




Images

OXCT2 antibodyWestern blot analysis of various lysates using OXCT2 Rabbit pAb (A14920) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence analysis of paraffin-embedded rat testis using OXCT2 Rabbit pAb (A14920) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffin-embedded mouse testis using OXCT2 Rabbit pAb (A14920) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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