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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Monoclonal antibody |
| Gene Name: | polybromo 1 |
| Gene Symbol: | PBRM1 |
| Synonyms: | PB1; RCC; BAF180; SMARCH1; PBRM1 |
| Gene ID: | 55193 |
| UniProt ID: | Q86U86 |
| Clone ID: | 9A8C4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 530-680 of human PBRM1 (Q86U86). |
| Dilution: | WB 1:1000-1:2000; IHC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1.5 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PBRM1 antibody |
Background
This locus encodes a subunit of ATP-dependent chromatin-remodeling complexes. The encoded protein has been identified as in integral component of complexes necessary for ligand-dependent transcriptional activation by nuclear hormone receptors. Mutations at this locus have been associated with primary clear cell renal cell carcinoma.
Images
 | Western blot analysis of various lysates using PBRM1 Rabbit mAb (A0334) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using PBRM1 Rabbit mAb (A0334) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human spleen using PBRM1 Rabbit mAb (A0334) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using PBRM1 Rabbit mAb (A0334) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
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