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PE Rabbit anti-Human IFN-gamma mAb (100 T)
| Reactivity: | Human |
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | PE. Ex:565nm. Em:574nm. |
| Gene Name: | interferon gamma |
| Gene Symbol: | IFNG |
| Synonyms: | IFG; IFI; IMD69 |
| Gene ID: | 3458 |
| UniProt ID: | P01579 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-166 of human IFN-gamma (NP_000610.2). |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-IFNG Antibody |
Background
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
Images
 | Flow cytometry: 1×10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were intracellularly-stained with PE Rabbit anti-Human IFN-gamma mAb (A27139,5 μl/Test,orange line) or PE Rabbit IgG isotype control (A24172,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 Human PBMC (negative control,left) and Human PBMC (treated with 50ng/mL PMA and 1ug/mL ionomycin for 4-6 hours, right) were intracellularly-stained with PE Rabbit anti-Human IFN-gamma mAb (A27139,2 μl/Test,orange line) or PE Rabbit IgG isotype control (A24172,2 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). Cells in the Lymphocytes gate were used for analysis. |
 | Flow cytometry: 1×10^6 293T (Transfection) cells were intracellularly-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human IFN-gamma mAb (A27139,5 μl/Test,right). |
 | Flow cytometry: 1×10^6 Human PBMC (treated with 50ng/mL PMA and 1ug/mL ionomycin for 4-6 hours) were intracellularly-stained with PE Rabbit IgG isotype control (A24172,5 μl/Test,left) or PE Rabbit anti-Human IFN-gamma mAb (A27139,5 μl/Test,right). Cells in the Lymphocytes gate were used for analysis. |
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