PEBP1 Rabbit pAb (20 μl)

PEBP1 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A0578
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IF/IC, IP, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: phosphatidylethanolamine binding protein 1
Gene Symbol: PEBP1
Synonyms: PBP; HCNP; PEBP; RKIP; HCNPpp; PEBP-1; HEL-210; HEL-S-34; HEL-S-96; PEBP1
Gene ID: 5037
UniProt ID: P30086
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human PEBP1 (NP_002558.1).
Dilution: WB 1:500-1:1000; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 0.37 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-PEBP1 antibody




Background

This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome.




Images

PEBP1 antibodyWestern blot analysis of various lysates using PEBP1 Rabbit pAb (A0578) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using PEBP1 Rabbit pAb (A0578) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PEBP1 Rabbit pAb (A0578) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using PEBP1 Rabbit pAb (A0578) at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 150 μg extracts of MCF-7 cells using 3 μg PEBP1 antibody (A0578). Western blot was performed from the immunoprecipitate using PEBP1 antibody (A0578) at a dilution of 1:500.



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