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| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PFKL rabbit polyclonal antibody |
Gene Name: | ATP-dependent 6-phosphofructokinase, liver type |
Synonyms: | ATP-PFK, PFK-L, PFK-B, PFKL, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B |
Immunogen: | Recombinant protein corresponding to Human PFKL |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Uniprot ID: | P17858 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Human | 1: 1000-1: 3000 | hepatocarcinoma, liver, prostate |
Background
PFKL is the Liver isozyme of PFK (Phosphofructokinase), which is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate by ATP. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway.
Images
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Immunohistochemistry of paraffin embedded human hepatocarcinoma using pfk1 (GB111136) at dilution of 1:1600 (400x lens) |
 | Immunohistochemistry of paraffin embedded human liver using pfk1 (GB111136) at dilution of 1:1600 (400x lens) |
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Immunohistochemistry of paraffin embedded human prostate using pfk1 (GB111136) at dilution of 1:1600 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |