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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | phosphoinositide-3-kinase regulatory subunit 4 |
| Gene Symbol: | PIK3R4 |
| Synonyms: | p150; VPS15; PIK3R4/VPS15 |
| Gene ID: | 30849 |
| UniProt ID: | Q99570 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human PIK3R4/VPS15 (NP_055417.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:100; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.54 mg/mL |
| Buffer: | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-PIK3R4 antibody |
Background
Predicted to enable protein serine/threonine kinase activity. Involved in positive regulation of phosphatidylinositol 3-kinase activity; receptor catabolic process; and regulation of cytokinesis. Located in late endosome and microtubule cytoskeleton.
Images
 | Western blot analysis of various lysates using PIK3R4/VPS15 Rabbit pAb (A15828) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunofluorescence analysis of NIH/3T3 cells using PIK3R4/VPS15 Rabbit pAb (A15828) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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