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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Pim-1 proto-oncogene, serine/threonine kinase |
| Gene Symbol: | PIM1 |
| Synonyms: | PIM; PIM1 |
| Gene ID: | 5292 |
| UniProt ID: | P11309 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human PIM1 (NP_001230115.1). |
| Dilution: | WB 1:1000-1:5000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.80 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-PIM1 antibody |
Background
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).
Images
 | Western blot analysis of various lysates using PIM1 Rabbit pAb (A14210) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from mouse kidney, using PIM1 Rabbit pAb (A14210) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using PIM1 Rabbit pAb (A14210) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M PBS buffer (pH 7.2±0.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord tissue using PIM1 Rabbit pAb (A14210) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0.01 M PBS buffer (pH 7.2±0.2) prior to IHC staining. |
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