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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Parkin RBR E3 ubiquitin protein ligase |
| Gene Symbol: | PRKN |
| Synonyms: | PDJ; AR-JP; LPRS2; PARK2; Parkin |
| Gene ID: | 5071 |
| UniProt ID: | O60260 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 81-280 of human Parkin (NP_004553.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.18 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PRKN antibody |
Background
The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.
Images
 | Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Parkin using Parkin Rabbit pAb (A0968) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry analysis of paraffin-embedded Human kidney using Parkin Rabbit pAb (A0968) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of paraffin-embedded mouse brain using Parkin Rabbit pAb (A0968) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. |
 | Immunofluorescence analysis of paraffin-embedded rat brain using Parkin Rabbit pAb (A0968) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform microwave antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. |
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