PSMD14 Rabbit pAb (20 μl)

PSMD14 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A10782
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: proteasome 26S subunit, non-ATPase 14
Gene Symbol: PSMD14
Synonyms: PAD1; POH1; RPN11; PSMD14
Gene ID: 10213
UniProt ID: O00487
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-300 of human PSMD14 (NP_005796.1).
Dilution: WB 1:500-1:2000; IF/IC 1:50-1:100
Purification Method: Affinity purification
Concentration: 2.37 mg/ml
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-PSMD14 antibody




Background

This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2.




Images

PSMD14 antibodyWestern blot analysis of various lysates using PSMD14 Rabbit pAb (A10782) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of NIH-3T3 cells using PSMD14 Rabbit pAb (A10782) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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