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Anti-Phospho-AMPK alpha 1 (T183) + AMPK alpha 2 (T172) Rabbit pAb (100 μl)
| Reactivity: | M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 5'-AMP-activated protein kinase catalytic subunit alpha-1/5'-AMP-activated protein kinase catalytic subunit alpha-2 |
Synonyms: | AMPK, AMPK alpha 2, AMPK subunit alpha 2, AMPK2, AMPKA2, AMPKα2, PRKAA, PRKAA2, HMGCR kinase, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human AMPK alpha 1 (T183) + AMPK alpha 2 (T172) |
Uniprot ID: | Q13131, P54646 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 62 kDa / 65 kDa |
Product Usage Information
WB | Mouse | 1: 300-1: 600 | C2C12 |
Background
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.
Images
| Western blot analysis of Phospho-AMPK alpha1 (T183) + AMPK alpha 2 (T172) GB114323 at dilution of 1: 300 Lane1: C2C12 untreated Lane2: C2C12 treated with 0.5mM AICAR for 30min The figure below was treated with lambda phosphatase, the figure above was untreated |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |