Detection range: | 15.6-1000pg/mL |
Sensitivity: | 6.2pg/mL |
Type: | Traditional EGF ELISA kit |
Gene Name: | epidermal growth factor |
Gene Symbol: | EGF |
Synonyms: | URG; Beta-Urogastrone; Pro-epidermal growth factor |
Species: | Pig |
Sample type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Experimental method: | Sandwich |
Shelf life: | 12 months |
Gene ID: | 397083 |
UniProt ID: | Q00968 |
Components: | 1. Pre-coated, ready to use 96-well strip plate 1 2. Plate sealer for 96 wells 2 3. Standard 2 4. Diluents buffer: 1×45 mL 5. Detection Reagent A: 1×120 μL 6. Detection Reagent B: 1×120 μL 7. TMB Substrate: 1×9 mL 8. Stop Solution: 1×6 mL 9. Wash Buffer (30× concentrate): 1×20 mL |
Documents: | Manual-Porcine EGF ELISA Kit |
Background
The gene EGF encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Images
Typical Standard Curve for Porcine EGF ELISA |
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