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| Reactivity: | Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Monoclonal antibody |
| Gene Name: | acyl-CoA synthetase short chain family member 2 |
| Gene Symbol: | ACSS2 |
| Synonyms: | ACS; ACSA; ACAS2; ACECS; AceCS1; dJ1161H23.1; ACSS2 |
| Gene ID: | 55902 |
| UniProt ID: | Q9NR19 |
| Clone ID: | 4N5V4 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ACSS2 (Q9NR19). |
| Dilution: | WB 1:1000-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 2 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ACSS2 antibody |
Background
This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using ACSS2 Rabbit mAb (A12334) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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