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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | AFG3 like matrix AAA peptidase subunit 2 |
| Gene Symbol: | AFG3L2 |
| Synonyms: | OPA12; SCA28; SPAX5; AFG3L2 |
| Gene ID: | 10939 |
| UniProt ID: | Q9Y4W6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 538-797 of human AFG3L2 (NP_006787.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.65 mg/mL |
| Buffer: | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-AFG3L2 antibody |
Background
This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.
Images
 | Western blot analysis of various lysates, using AFG3L2 Rabbit pAb (A15393) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using AFG3L2 Rabbit pAb (A15393) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg AFG3L2 antibody (A15393). Western blot was performed from the immunoprecipitate using AFG3L2 antibody (A15393) at a dilution of 1:1000. |
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