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beta 2 Microglobulin Rabbit mAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | beta-2-microglobulin |
| Gene Symbol: | B2M |
| Synonyms: | IMD43; beta 2 Microglobulin |
| Gene ID: | 567 |
| UniProt ID: | P61769 |
| Clone ID: | 5F0O7 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 40-119 of human beta 2 Microglobulin (P61769). |
| Dilution: | WB 1:3000-1:18000; IHC 1:1000-1:5000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.42 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-B2M antibody |
Background
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Images
 | Western blot analysis of various lysates using beta 2 Microglobulin Rabbit mAb (A11642)at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): Daudi Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using beta 2 Microglobulin Rabbit mAb (A11642) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using beta 2 Microglobulin Rabbit mAb (A11642) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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