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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | items found |
| Gene Symbol: | CAS9 |
| Synonyms: | CAS9 |
| Gene ID: | 901176 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 450-730 of streptococcus pyogenes m1 gas CAS9 (NP_269215.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Buffer: | Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CAS9 antibody |
Background
Staphylococcus aureus Cas9 (SaCas9) is an RNA-guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its small size facilitates in vivo delivery for genome editing in various organisms.
Images
 | Western blot analysis of extracts of 293T cells and 293T transfected with CAS9 protein, using CAS9 Rabbit pAb (A14997) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |
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