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| Reactivity: | Human |
| Applications: | WB, IHC, FC (intra), ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | CD247 molecule |
| Gene Symbol: | CD247 |
| Synonyms: | T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3ZETA; CD3-ZETA |
| Gene ID: | 919 |
| UniProt ID: | P20963 |
| Clone ID: | 1T5R7 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 65-164 of human CD3H (P20963). |
| Dilution: | WB 1:1000-1:2000; IHC 1:50-1:200; FC (intra) 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.60 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD247 antibody |
Background
The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from Jurkat cells, using CD3H Rabbit mAb (A11157) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD3H Rabbit mAb (A11157) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using CD3H Rabbit mAb (A11157) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Flow cytometry:1X10^6 Raji cells (negative control,left) and Jurkat cells (right) were intracellularly-stained with CD3H Rabbit mAb(A11157, 10 μg/mL,orange line) or Rabbit IgG isotype control (AC042, 10 μg/mL,blue line),followed by FITC conjugated goat anti-rabbit pAb(1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line). |
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