CD34 Rabbit pAb (20 μl)

CD34 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A13929
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IHC, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: CD34 molecule
Gene Symbol: CD34
Synonyms: CD34; CD34 molecule; GIG3; MORT1
Gene ID: 947
UniProt ID: P28906
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 32-290 of human CD34 (NP_001020280.1).
Dilution: WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 1.86 mg/ml
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-CD34 antibody




Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.




Images

DFFB antibodyWestern blot analysis of various lysates using CD34 Rabbit pAb (A13929) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using CD34 Rabbit pAb (A13929) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CD34 Rabbit pAb (A13929) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta using CD34 Rabbit pAb (A13929) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of TF-1 cells using CD34 Rabbit pAb (A13929) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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