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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ceramide transporter 1 |
| Gene Symbol: | CERT1 |
| Synonyms: | CERT; GPBP; CERTL; MRD34; STARD11; COL4A3BP |
| Gene ID: | 10087 |
| UniProt ID: | Q9Y5P4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 369-598 of human COL4A3BP (NP_112729.1). |
| Dilution: | WB 1:200-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.53 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CERT1 antibody |
Background
This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of various lysates using COL4A3BP Rabbit pAb (A15376) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of L929 cells using COL4A3BP Rabbit pAb (A15376) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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