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Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | casein kinase 2 alpha 1 |
| Gene Symbol: | CSNK2A1 |
| Synonyms: | CKII; Cka1; Cka2; CK2A1; OCNDS; Casein Kinase 2 alpha (CSNK2A1) |
| Gene ID: | 1457 |
| UniProt ID: | P68400 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-391 of human Casein Kinase 2 alpha (CSNK2A1) (NP_001886.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.23 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CSNK2A1 antibody |
Background
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene.
Images
 | Western blot analysis of various lysates using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Western blot analysis of lysates from SGC-7901 cells using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat liver using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Casein Kinase 2 alpha (CSNK2A1) Rabbit pAb (A1014) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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