Casein Kinase 2 beta (CSNK2B) Rabbit pAb (20 μl)

Casein Kinase 2 beta (CSNK2B) Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A14722
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IHC, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: casein kinase 2 beta
Gene Symbol: CSNK2B
Synonyms: G5A; CK2B; CK2N; Ckb1; Ckb2; CSK2B; POBINDS; Casein Kinase 2 beta (CSNK2B)
Gene ID: 1460
UniProt ID: P67870
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human Casein Kinase 2 beta (Casein Kinase 2 beta (CSNK2B)) (NP_001311.3).
Dilution: WB 1:500-1:2000; IHC 1:50-1:100; IF/IC 1:50-1:100
Purification Method: Affinity purification
Concentration: 0.78 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-CSNK2B antibody




Background

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene.




Images

LGALS3 antibodyWestern blot analysis of various lysates using Casein Kinase 2 beta (Casein Kinase 2 beta (CSNK2B)) Rabbit pAb (A14722) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of NIH-3T3 cells using Casein Kinase 2 beta (CSNK2B) Rabbit pAb (A14722) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Casein Kinase 2 beta (CSNK2B) Rabbit pAb (A14722) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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