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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | cytochrome b-245 beta chain |
| Gene Symbol: | CYBB |
| Synonyms: | CGD; CGDX; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX; NOX2/gp91phox |
| Gene ID: | 1536 |
| UniProt ID: | P04839 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 101-200 of human NOX2/gp91phox (NP_000388.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.94 mg/mL |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CYBB antibody |
Background
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Images
 | Western blot analysis of lysates from RAW 264.7 cells, using NOX2/gp91phox Rabbit pAb (A12430) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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