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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | dystroglycan 1 |
| Gene Symbol: | DAG1 |
| Synonyms: | A3a; DAG; AGRNR; 156DAG; MDDGA9; MDDGC7; MDDGC9; LGMDR16; DAG1 |
| Gene ID: | 1605 |
| UniProt ID: | Q14118 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 776-895 of human DAG1 (NP_001171108.1). |
| Dilution: | WB 1:1000-1:5000; IF/IC 1:20-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 0.87 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-DAG1 antibody |
Background
This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein.
Images
 | Western blot analysis of lysates from Mouse heart, using DAG1 Rabbit pAb (A10076) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunofluorescence analysis of HeLa cells using DAG1 Rabbit pAb(A10076) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg DAG1 antibody (A10076). Western blot was performed from the immunoprecipitate using DAG1 antibody (A10076) at a dilution of 1:1000. |
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