Reactivity: | Human, Mouse |
Applications: | WB, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Monoclonal antibody |
Gene Name: | death associated protein kinase 1 |
Gene Symbol: | DAPK1 |
Synonyms: | DAPK; ROCO3; DAP Kinase 1 (DAPK1) |
Gene ID: | 1612 |
UniProt ID: | P53355 |
Clone ID: | 3E10G3 |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human DAP Kinase 1 (DAPK1) (P53355). |
Dilution: | WB 1:500-1:1000 |
Purification Method: | Affinity purification |
Concentration: | 1.5 mg/mL |
Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Documents: | Manual-DAPK1 antibody |
Background
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants.
Images
![]() | Western blot analysis of various lysates using DAP Kinase 1 (DAPK1) Rabbit mAb (A11459) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
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