Your shopping cart is empty!
| Reactivity: | Human |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | dickkopf WNT signaling pathway inhibitor 2 |
| Gene Symbol: | DKK2 |
| Synonyms: | DKK-2; DKK2 |
| Gene ID: | 27123 |
| UniProt ID: | Q9UBU2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DKK2 (NP_055236.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 2.84 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-DKK2 antibody |
Background
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6).
Images
 | Western blot analysis of various lysates using DKK2 Rabbit pAb (A14874) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of paraffin-embedded human skin cancer using DKK2 Rabbit pAb (A14874) at dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
You may also be interested in:
