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| Reactivity: | Human, Mouse |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | dihydrolipoamide dehydrogenase |
| Gene Symbol: | DLD |
| Synonyms: | E3; LAD; DLDD; DLDH; GCSL; PHE3; OGDC-E3; DLDH/DLD |
| Gene ID: | 1738 |
| UniProt ID: | P09622 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human DLDH/DLDH/DLDH/DLD (NP_000099.2). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:500; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.42 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-DLD antibody |
Background
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using DLDH/DLD Rabbit pAb (A13296) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using DLDH/DLDH/DLDH/DLD Rabbit pAb (A13296) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human placenta using DLDH/DLDH/DLDH/DLD Rabbit pAb (A13296) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of HeLa cells using DLDH/DLD Rabbit pAb (A13296). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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