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| Reactivity: | Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | deoxyribonuclease 1 like 1 |
| Gene Symbol: | DNASE1L1 |
| Synonyms: | XIB; G4.8; DNL1L; DNASEX; DNAS1L1; DNASE1L1 |
| Gene ID: | 1774 |
| UniProt ID: | P49184 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human DNASE1L1 (NP_006721.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.98 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-DNASE1L1 antibody |
Background
This gene encodes a deoxyribonuclease protein that shows high sequence similarity to DNase I. The encoded protein is localized to the endoplasmic reticulum and modified by N-linked glycosylation. Alternate transcriptional splice variants encoding the same protein have been observed.
Images
 | Western blot analysis of lysates from mouse heart, using DNASE1L1 Rabbit pAb (A13150) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using DNASE1L1 Rabbit pAb (A13150) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using DNASE1L1 Rabbit pAb (A13150) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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